Construct: shRNA TRCN0000198048

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178672.3-801s1c1
DNA Barcode:
CTCAGTTCTCTGTGTGAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scfd2 (212986)
Gene Description:
Sec1 family domain containing 2
Transcript:
RefSeq NM_178672.3 (NON-CURRENT)
Match location:
Position 801 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198048)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGTTCTCTGTGTGAACAT
Hairpin Sequence:
5'-CCGG-CTCAGTTCTCTGTGTGAACAT-CTCGAG-ATGTTCACACAGAGAACTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGTTCTCTGTGTGAACATCTCGAGATGTTCACACAGAGAACTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGTTCTCTGTGTGAACATCTCGAGATGTTCACACAGAGAACTGAG-3'

Other clones with same target sequence:

(none)