Construct: shRNA TRCN0000198094

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145505.2-1415s1c1
DNA Barcode:
CCAGGACTACAACTTAGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam160b1 (226252)
Gene Description:
family with sequence similarity 160, member B1
Transcript:
RefSeq NM_145505.2 (NON-CURRENT)
Match location:
Position 1415 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198094)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGACTACAACTTAGTGAA
Hairpin Sequence:
5'-CCGG-CCAGGACTACAACTTAGTGAA-CTCGAG-TTCACTAAGTTGTAGTCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGACTACAACTTAGTGAACTCGAGTTCACTAAGTTGTAGTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGACTACAACTTAGTGAACTCGAGTTCACTAAGTTGTAGTCCTGG-3'

Other clones with same target sequence:

(none)