Construct: shRNA TRCN0000198095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145959.1-1813s1c1
DNA Barcode:
CATCGCTAGTCTCCATTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam91a1 (210998)
Gene Description:
family with sequence similarity 91, member A1
Transcript:
RefSeq NM_145959.1 (NON-CURRENT)
Match location:
Position 1813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGCTAGTCTCCATTTGTA
Hairpin Sequence:
5'-CCGG-CATCGCTAGTCTCCATTTGTA-CTCGAG-TACAAATGGAGACTAGCGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGCTAGTCTCCATTTGTACTCGAGTACAAATGGAGACTAGCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGCTAGTCTCCATTTGTACTCGAGTACAAATGGAGACTAGCGATG-3'

Other clones with same target sequence:

  1. TRCN0000279146