Construct: shRNA TRCN0000198170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029610.1-746s1c1
DNA Barcode:
CCAAACCAGTGTATCTGAAGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lyrm1 (73919)
Gene Description:
LYR motif containing 1
Transcript:
RefSeq NM_029610.1 (NON-CURRENT)
Match location:
Position 746 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCAGTGTATCTGAAGT
Hairpin Sequence:
5'-CCGG-CCAAACCAGTGTATCTGAAGT-CTCGAG-ACTTCAGATACACTGGTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCAGTGTATCTGAAGTCTCGAGACTTCAGATACACTGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCAGTGTATCTGAAGTCTCGAGACTTCAGATACACTGGTTTGG-3'

Other clones with same target sequence:

(none)