Construct: shRNA TRCN0000198173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033072.1-720s1c1
DNA Barcode:
CAGTGAAAGGTACAGAGCAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mbd6 (110962)
Gene Description:
methyl-CpG binding domain protein 6
Transcript:
RefSeq NM_033072.1 (NON-CURRENT)
Match location:
Position 720 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGAAAGGTACAGAGCAAT
Hairpin Sequence:
5'-CCGG-CAGTGAAAGGTACAGAGCAAT-CTCGAG-ATTGCTCTGTACCTTTCACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGAAAGGTACAGAGCAATCTCGAGATTGCTCTGTACCTTTCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGAAAGGTACAGAGCAATCTCGAGATTGCTCTGTACCTTTCACTG-3'

Other clones with same target sequence:

(none)