Construct: shRNA TRCN0000198199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025772.3-626s1c1
DNA Barcode:
CCTTCAAAGCTGAACTCGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dtnbp1 (94245)
Gene Description:
dystrobrevin binding protein 1
Transcript:
RefSeq NM_025772.3 (NON-CURRENT)
Match location:
Position 626 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAAAGCTGAACTCGATA
Hairpin Sequence:
5'-CCGG-CCTTCAAAGCTGAACTCGATA-CTCGAG-TATCGAGTTCAGCTTTGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAAAGCTGAACTCGATACTCGAGTATCGAGTTCAGCTTTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAAAGCTGAACTCGATACTCGAGTATCGAGTTCAGCTTTGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000278790