Construct: shRNA TRCN0000198242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175293.2-887s1c1
DNA Barcode:
GCTTGAGATGTGTTTAGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D630023F18Rik (98303)
Gene Description:
RIKEN cDNA D630023F18 gene
Transcript:
RefSeq NM_175293.2 (NON-CURRENT)
Match location:
Position 887 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGAGATGTGTTTAGTCTT
Hairpin Sequence:
5'-CCGG-GCTTGAGATGTGTTTAGTCTT-CTCGAG-AAGACTAAACACATCTCAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGAGATGTGTTTAGTCTTCTCGAGAAGACTAAACACATCTCAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGAGATGTGTTTAGTCTTCTCGAGAAGACTAAACACATCTCAAGC-3'

Other clones with same target sequence:

(none)