Construct: shRNA TRCN0000198252

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175184.3-978s1c1
DNA Barcode:
GCGGCTCTGCATTAAGTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mvb12b (72543)
Gene Description:
multivesicular body subunit 12B
Transcript:
RefSeq NM_175184.3 (NON-CURRENT)
Match location:
Position 978 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198252)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTCTGCATTAAGTTTAT
Hairpin Sequence:
5'-CCGG-GCGGCTCTGCATTAAGTTTAT-CTCGAG-ATAAACTTAATGCAGAGCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTCTGCATTAAGTTTATCTCGAGATAAACTTAATGCAGAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTCTGCATTAAGTTTATCTCGAGATAAACTTAATGCAGAGCCGC-3'

Other clones with same target sequence:

(none)