Construct: shRNA TRCN0000198261

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130885.1-1734s1c1
DNA Barcode:
GCCTGGATTCATAGTAGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Oxr1 (170719)
Gene Description:
oxidation resistance 1
Transcript:
RefSeq NM_130885.1 (NON-CURRENT)
Match location:
Position 1734 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198261)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGATTCATAGTAGTCAA
Hairpin Sequence:
5'-CCGG-GCCTGGATTCATAGTAGTCAA-CTCGAG-TTGACTACTATGAATCCAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGATTCATAGTAGTCAACTCGAGTTGACTACTATGAATCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGATTCATAGTAGTCAACTCGAGTTGACTACTATGAATCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000346008