Construct: shRNA TRCN0000198355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175209.1-319s1c1
DNA Barcode:
GCTTAGCAAGTGCATCACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A730049H05Rik (74516)
Gene Description:
RIKEN cDNA A730049H05 gene
Transcript:
RefSeq NM_175209.1 (NON-CURRENT)
Match location:
Position 319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAGCAAGTGCATCACAAA
Hairpin Sequence:
5'-CCGG-GCTTAGCAAGTGCATCACAAA-CTCGAG-TTTGTGATGCACTTGCTAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAGCAAGTGCATCACAAACTCGAGTTTGTGATGCACTTGCTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAGCAAGTGCATCACAAACTCGAGTTTGTGATGCACTTGCTAAGC-3'

Other clones with same target sequence:

(none)