Construct: shRNA TRCN0000198458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144824.1-543s1c1
DNA Barcode:
GTTTCTGAACATGCGAGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wrap53 (216853)
Gene Description:
WD repeat containing, antisense to Trp53
Transcript:
RefSeq NM_144824.1 (NON-CURRENT)
Match location:
Position 543 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCTGAACATGCGAGTCTT
Hairpin Sequence:
5'-CCGG-GTTTCTGAACATGCGAGTCTT-CTCGAG-AAGACTCGCATGTTCAGAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCTGAACATGCGAGTCTTCTCGAGAAGACTCGCATGTTCAGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCTGAACATGCGAGTCTTCTCGAGAAGACTCGCATGTTCAGAAAC-3'

Other clones with same target sequence:

  1. TRCN0000328525