Construct: shRNA TRCN0000198467

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028162.2-3256s1c1
DNA Barcode:
GCAGGGTTTCAGATTAGGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d5 (72238)
Gene Description:
TBC1 domain family, member 5
Transcript:
RefSeq NM_028162.2 (NON-CURRENT)
Match location:
Position 3256 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198467)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGGTTTCAGATTAGGAAT
Hairpin Sequence:
5'-CCGG-GCAGGGTTTCAGATTAGGAAT-CTCGAG-ATTCCTAATCTGAAACCCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGGTTTCAGATTAGGAATCTCGAGATTCCTAATCTGAAACCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGGTTTCAGATTAGGAATCTCGAGATTCCTAATCTGAAACCCTGC-3'

Other clones with same target sequence:

(none)