Construct: shRNA TRCN0000198483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026247.1-182s1c1
DNA Barcode:
GTCATTCACTCTGGATGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alg13 (67574)
Gene Description:
asparagine-linked glycosylation 13
Transcript:
RefSeq NM_026247.1 (NON-CURRENT)
Match location:
Position 182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATTCACTCTGGATGTTTA
Hairpin Sequence:
5'-CCGG-GTCATTCACTCTGGATGTTTA-CTCGAG-TAAACATCCAGAGTGAATGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATTCACTCTGGATGTTTACTCGAGTAAACATCCAGAGTGAATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATTCACTCTGGATGTTTACTCGAGTAAACATCCAGAGTGAATGAC-3'

Other clones with same target sequence:

(none)