Construct: shRNA TRCN0000198576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144901.2-752s1c1
DNA Barcode:
GAAGAACGAATGAACGGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csde1 (229663)
Gene Description:
cold shock domain containing E1, RNA binding
Transcript:
RefSeq NM_144901.2 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAACGAATGAACGGACAA
Hairpin Sequence:
5'-CCGG-GAAGAACGAATGAACGGACAA-CTCGAG-TTGTCCGTTCATTCGTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAACGAATGAACGGACAACTCGAGTTGTCCGTTCATTCGTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAACGAATGAACGGACAACTCGAGTTGTCCGTTCATTCGTTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000297097