Construct: shRNA TRCN0000198589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026312.2-491s1c1
DNA Barcode:
CGGGAAGGCTATAATAAAGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbdc1 (67683)
Gene Description:
polysaccharide biosynthesis domain containing 1
Transcript:
RefSeq NM_026312.2 (NON-CURRENT)
Match location:
Position 491 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAAGGCTATAATAAAGCA
Hairpin Sequence:
5'-CCGG-CGGGAAGGCTATAATAAAGCA-CTCGAG-TGCTTTATTATAGCCTTCCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAAGGCTATAATAAAGCACTCGAGTGCTTTATTATAGCCTTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAAGGCTATAATAAAGCACTCGAGTGCTTTATTATAGCCTTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000338120