Construct: shRNA TRCN0000198598

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183110.1-498s1c1
DNA Barcode:
CCCACACTACAGAAAGCAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930543L23Rik (75171)
Gene Description:
RIKEN cDNA 4930543L23 gene
Transcript:
RefSeq NM_183110.1 (NON-CURRENT)
Match location:
Position 498 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198598)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACACTACAGAAAGCAAGA
Hairpin Sequence:
5'-CCGG-CCCACACTACAGAAAGCAAGA-CTCGAG-TCTTGCTTTCTGTAGTGTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACACTACAGAAAGCAAGACTCGAGTCTTGCTTTCTGTAGTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACACTACAGAAAGCAAGACTCGAGTCTTGCTTTCTGTAGTGTGGG-3'

Other clones with same target sequence:

(none)