Construct: shRNA TRCN0000198606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175176.2-776s1c1
DNA Barcode:
CCTTGCTACAGGTGCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4922501L14Rik (71845)
Gene Description:
RIKEN cDNA 4922501L14 gene
Transcript:
RefSeq NM_175176.2 (NON-CURRENT)
Match location:
Position 776 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTACAGGTGCATTATT
Hairpin Sequence:
5'-CCGG-CCTTGCTACAGGTGCATTATT-CTCGAG-AATAATGCACCTGTAGCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTACAGGTGCATTATTCTCGAGAATAATGCACCTGTAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTACAGGTGCATTATTCTCGAGAATAATGCACCTGTAGCAAGG-3'

Other clones with same target sequence:

(none)