Construct: shRNA TRCN0000198623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144555.1-408s1c1
DNA Barcode:
CTCACTTTCGGGAGTCTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lncpint (232685)
Gene Description:
long non-protein coding RNA, Trp53 induced transcript
Transcript:
RefSeq NM_144555.1 (NON-CURRENT)
Match location:
Position 408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACTTTCGGGAGTCTCTTA
Hairpin Sequence:
5'-CCGG-CTCACTTTCGGGAGTCTCTTA-CTCGAG-TAAGAGACTCCCGAAAGTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACTTTCGGGAGTCTCTTACTCGAGTAAGAGACTCCCGAAAGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACTTTCGGGAGTCTCTTACTCGAGTAAGAGACTCCCGAAAGTGAG-3'

Other clones with same target sequence:

(none)