Construct: shRNA TRCN0000198626

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172850.1-1857s1c1
DNA Barcode:
CCGTTGGATGACCATCACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankmy1 (241158)
Gene Description:
ankyrin repeat and MYND domain containing 1
Transcript:
RefSeq NM_172850.1 (NON-CURRENT)
Match location:
Position 1857 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198626)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTGGATGACCATCACATT
Hairpin Sequence:
5'-CCGG-CCGTTGGATGACCATCACATT-CTCGAG-AATGTGATGGTCATCCAACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTGGATGACCATCACATTCTCGAGAATGTGATGGTCATCCAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTGGATGACCATCACATTCTCGAGAATGTGATGGTCATCCAACGG-3'

Other clones with same target sequence:

(none)