Construct: shRNA TRCN0000198627

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175488.3-520s1c1
DNA Barcode:
CCACGAGTTTATTAACGACCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccdc38 (237465)
Gene Description:
coiled-coil domain containing 38
Transcript:
RefSeq NM_175488.3 (NON-CURRENT)
Match location:
Position 520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198627)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGAGTTTATTAACGACCA
Hairpin Sequence:
5'-CCGG-CCACGAGTTTATTAACGACCA-CTCGAG-TGGTCGTTAATAAACTCGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGAGTTTATTAACGACCACTCGAGTGGTCGTTAATAAACTCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGAGTTTATTAACGACCACTCGAGTGGTCGTTAATAAACTCGTGG-3'

Other clones with same target sequence:

(none)