Construct: shRNA TRCN0000198652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199467.1-1553s1c1
DNA Barcode:
CCTGTCGAGAATCTGAACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mms22l (212377)
Gene Description:
MMS22-like, DNA repair protein
Transcript:
RefSeq NM_199467.1 (NON-CURRENT)
Match location:
Position 1553 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCGAGAATCTGAACTTA
Hairpin Sequence:
5'-CCGG-CCTGTCGAGAATCTGAACTTA-CTCGAG-TAAGTTCAGATTCTCGACAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCGAGAATCTGAACTTACTCGAGTAAGTTCAGATTCTCGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCGAGAATCTGAACTTACTCGAGTAAGTTCAGATTCTCGACAGG-3'

Other clones with same target sequence:

(none)