Construct: shRNA TRCN0000198681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030256.1-494s1c1
DNA Barcode:
CTCATGAACCTGCAGAACATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl9l (80288)
Gene Description:
B cell CLL/lymphoma 9-like
Transcript:
RefSeq NM_030256.1 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATGAACCTGCAGAACATG
Hairpin Sequence:
5'-CCGG-CTCATGAACCTGCAGAACATG-CTCGAG-CATGTTCTGCAGGTTCATGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATGAACCTGCAGAACATGCTCGAGCATGTTCTGCAGGTTCATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATGAACCTGCAGAACATGCTCGAGCATGTTCTGCAGGTTCATGAG-3'

Other clones with same target sequence:

  1. TRCN0000277574