Construct: shRNA TRCN0000198850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029404.1-3077s1c1
DNA Barcode:
CTCCTCAAGCTTCAAAGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf14 (75725)
Gene Description:
PHD finger protein 14
Transcript:
RefSeq NM_029404.1 (NON-CURRENT)
Match location:
Position 3077 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTCAAGCTTCAAAGACTT
Hairpin Sequence:
5'-CCGG-CTCCTCAAGCTTCAAAGACTT-CTCGAG-AAGTCTTTGAAGCTTGAGGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTCAAGCTTCAAAGACTTCTCGAGAAGTCTTTGAAGCTTGAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTCAAGCTTCAAAGACTTCTCGAGAAGTCTTTGAAGCTTGAGGAG-3'

Other clones with same target sequence:

(none)