Construct: shRNA TRCN0000198900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177756.3-1670s1c1
DNA Barcode:
GTAAATCTCGTCGAAGCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Colgalt2 (269132)
Gene Description:
collagen beta(1-O)galactosyltransferase 2
Transcript:
RefSeq NM_177756.3 (NON-CURRENT)
Match location:
Position 1670 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAAATCTCGTCGAAGCTGAT
Hairpin Sequence:
5'-CCGG-GTAAATCTCGTCGAAGCTGAT-CTCGAG-ATCAGCTTCGACGAGATTTAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAAATCTCGTCGAAGCTGATCTCGAGATCAGCTTCGACGAGATTTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAAATCTCGTCGAAGCTGATCTCGAGATCAGCTTCGACGAGATTTAC-3'

Other clones with same target sequence:

(none)