Construct: shRNA TRCN0000198907

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027614.1-1955s1c1
DNA Barcode:
GCTGCACCAGATCTAGAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr20rt (70948)
Gene Description:
WD repeat domain 20, retrogene
Transcript:
RefSeq NM_027614.1 (CURRENT)
Match location:
Position 1955 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198907)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCACCAGATCTAGAACTT
Hairpin Sequence:
5'-CCGG-GCTGCACCAGATCTAGAACTT-CTCGAG-AAGTTCTAGATCTGGTGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCACCAGATCTAGAACTTCTCGAGAAGTTCTAGATCTGGTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCACCAGATCTAGAACTTCTCGAGAAGTTCTAGATCTGGTGCAGC-3'

Other clones with same target sequence:

(none)