Construct: shRNA TRCN0000198916

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026252.2-385s1c1
DNA Barcode:
GAAGCTGGAATACTGCCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cpeb4 (67579)
Gene Description:
cytoplasmic polyadenylation element binding protein 4
Transcript:
RefSeq NM_026252.2 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198916)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCTGGAATACTGCCTGAA
Hairpin Sequence:
5'-CCGG-GAAGCTGGAATACTGCCTGAA-CTCGAG-TTCAGGCAGTATTCCAGCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCTGGAATACTGCCTGAACTCGAGTTCAGGCAGTATTCCAGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCTGGAATACTGCCTGAACTCGAGTTCAGGCAGTATTCCAGCTTC-3'

Other clones with same target sequence:

(none)