Construct: shRNA TRCN0000198923

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009951.1-283s1c1
DNA Barcode:
GCGCTTTACAGATACTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep44 (382010)
Gene Description:
centrosomal protein 44
Transcript:
RefSeq NM_001009951.1 (CURRENT)
Match location:
Position 283 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000198923)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTTACAGATACTGTCTA
Hairpin Sequence:
5'-CCGG-GCGCTTTACAGATACTGTCTA-CTCGAG-TAGACAGTATCTGTAAAGCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTTACAGATACTGTCTACTCGAGTAGACAGTATCTGTAAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTTACAGATACTGTCTACTCGAGTAGACAGTATCTGTAAAGCGC-3'

Other clones with same target sequence:

(none)