Construct: shRNA TRCN0000198947
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_026844.2-146s1c1
- DNA Barcode:
- GAAGAATGCAACGTCCTGATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Cmc2 (66531)
- Gene Description:
- COX assembly mitochondrial protein 2
- Transcript:
- RefSeq NM_026844.2 (NON-CURRENT)
- Match location:
- Position 146 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000198947)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAAGAATGCAACGTCCTGATT
- Hairpin Sequence:
-
5'-CCGG-GAAGAATGCAACGTCCTGATT-CTCGAG-AATCAGGACGTTGCATTCTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAAGAATGCAACGTCCTGATTCTCGAGAATCAGGACGTTGCATTCTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAAGAATGCAACGTCCTGATTCTCGAGAATCAGGACGTTGCATTCTTC-3'
Other clones with same target sequence:
(none)
