Construct: shRNA TRCN0000199055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004672.3-2887s1c1
DNA Barcode:
CAAGCCTTTCTCCTCCGAACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K6 (9064)
Gene Description:
mitogen-activated protein kinase kinase kinase 6
Transcript:
RefSeq NM_004672.3 (NON-CURRENT)
Match location:
Position 2887 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCCTTTCTCCTCCGAACT
Hairpin Sequence:
5'-CCGG-CAAGCCTTTCTCCTCCGAACT-CTCGAG-AGTTCGGAGGAGAAAGGCTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCCTTTCTCCTCCGAACTCTCGAGAGTTCGGAGGAGAAAGGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCCTTTCTCCTCCGAACTCTCGAGAGTTCGGAGGAGAAAGGCTTG-3'

Other clones with same target sequence:

  1. TRCN0000352612