Construct: shRNA TRCN0000199065

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002645.1-4593s1c1
DNA Barcode:
CCACCCTTTACTTCGTGATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3C2A (5286)
Gene Description:
phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
Transcript:
RefSeq NM_002645.1 (NON-CURRENT)
Match location:
Position 4593 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199065)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACCCTTTACTTCGTGATGA
Hairpin Sequence:
5'-CCGG-CCACCCTTTACTTCGTGATGA-CTCGAG-TCATCACGAAGTAAAGGGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACCCTTTACTTCGTGATGACTCGAGTCATCACGAAGTAAAGGGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACCCTTTACTTCGTGATGACTCGAGTCATCACGAAGTAAAGGGTGG-3'

Other clones with same target sequence:

(none)