Construct: shRNA TRCN0000199079

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004442.6-1451s1c1
DNA Barcode:
CCATCGGCAGTGTCCATCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHB2 (2048)
Gene Description:
EPH receptor B2
Transcript:
RefSeq NM_004442.6 (NON-CURRENT)
Match location:
Position 1451 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199079)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCGGCAGTGTCCATCATG
Hairpin Sequence:
5'-CCGG-CCATCGGCAGTGTCCATCATG-CTCGAG-CATGATGGACACTGCCGATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCGGCAGTGTCCATCATGCTCGAGCATGATGGACACTGCCGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCGGCAGTGTCCATCATGCTCGAGCATGATGGACACTGCCGATGG-3'

Other clones with same target sequence:

(none)