Construct: shRNA TRCN0000199092
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_047355.9-1158s1c1
- DNA Barcode:
- CGAGGAGCTTTCACTAGATGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCLK3 (85443)
- Gene Description:
- doublecortin like kinase 3
- Transcript:
- RefSeq XM_047355.9 (NON-CURRENT)
- Match location:
- Position 1158 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGAGGAGCTTTCACTAGATGA
- Hairpin Sequence:
-
5'-CCGG-CGAGGAGCTTTCACTAGATGA-CTCGAG-TCATCTAGTGAAAGCTCCTCG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGAGGAGCTTTCACTAGATGACTCGAGTCATCTAGTGAAAGCTCCTCGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGAGGAGCTTTCACTAGATGACTCGAGTCATCTAGTGAAAGCTCCTCG-3'
Other clones with same target sequence:
(none)
