Construct: shRNA TRCN0000199124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145001.1-670s1c1
DNA Barcode:
CTGGCTCTCCTACAAGTCCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK32A (202374)
Gene Description:
serine/threonine kinase 32A
Transcript:
RefSeq NM_145001.1 (NON-CURRENT)
Match location:
Position 670 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCTCTCCTACAAGTCCCA
Hairpin Sequence:
5'-CCGG-CTGGCTCTCCTACAAGTCCCA-CTCGAG-TGGGACTTGTAGGAGAGCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCTCTCCTACAAGTCCCACTCGAGTGGGACTTGTAGGAGAGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCTCTCCTACAAGTCCCACTCGAGTGGGACTTGTAGGAGAGCCAG-3'

Other clones with same target sequence:

(none)