Construct: shRNA TRCN0000199143

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012398.1-175s1c1
DNA Barcode:
CCAACAGAGGTTCTGTCCATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5K1C (23396)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
Transcript:
RefSeq NM_012398.1 (NON-CURRENT)
Match location:
Position 175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199143)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACAGAGGTTCTGTCCATG
Hairpin Sequence:
5'-CCGG-CCAACAGAGGTTCTGTCCATG-CTCGAG-CATGGACAGAACCTCTGTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACAGAGGTTCTGTCCATGCTCGAGCATGGACAGAACCTCTGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACAGAGGTTCTGTCCATGCTCGAGCATGGACAGAACCTCTGTTGG-3'

Other clones with same target sequence:

(none)