Construct: shRNA TRCN0000199162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002627.3-1829s1c1
DNA Barcode:
CCGGAGCTGATGCCGCATACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PFKP (5214)
Gene Description:
phosphofructokinase, platelet
Transcript:
RefSeq NM_002627.3 (NON-CURRENT)
Match location:
Position 1829 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAGCTGATGCCGCATACA
Hairpin Sequence:
5'-CCGG-CCGGAGCTGATGCCGCATACA-CTCGAG-TGTATGCGGCATCAGCTCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAGCTGATGCCGCATACACTCGAGTGTATGCGGCATCAGCTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAGCTGATGCCGCATACACTCGAGTGTATGCGGCATCAGCTCCGG-3'

Other clones with same target sequence:

(none)