Construct: shRNA TRCN0000199176

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020680.2-996s1c1
DNA Barcode:
CGCCTTCGAGTTCGGCAATGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL1 (57410)
Gene Description:
SCY1 like pseudokinase 1
Transcript:
RefSeq NM_020680.2 (NON-CURRENT)
Match location:
Position 996 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199176)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTCGAGTTCGGCAATGC
Hairpin Sequence:
5'-CCGG-CGCCTTCGAGTTCGGCAATGC-CTCGAG-GCATTGCCGAACTCGAAGGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTCGAGTTCGGCAATGCCTCGAGGCATTGCCGAACTCGAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTCGAGTTCGGCAATGCCTCGAGGCATTGCCGAACTCGAAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000352681