Construct: shRNA TRCN0000199184

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133494.1-615s1c1
DNA Barcode:
CCACAGCTGCACATTCTTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK7 (140609)
Gene Description:
NIMA related kinase 7
Transcript:
RefSeq NM_133494.1 (NON-CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199184)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCTGCACATTCTTTAG
Hairpin Sequence:
5'-CCGG-CCACAGCTGCACATTCTTTAG-CTCGAG-CTAAAGAATGTGCAGCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCTGCACATTCTTTAGCTCGAGCTAAAGAATGTGCAGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCTGCACATTCTTTAGCTCGAGCTAAAGAATGTGCAGCTGTGG-3'

Other clones with same target sequence:

(none)