Construct: shRNA TRCN0000199283

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000154.1-494s1c1
DNA Barcode:
CCTTGGAAGTGGCCACGTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GALK1 (2584)
Gene Description:
galactokinase 1
Transcript:
RefSeq NM_000154.1 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199283)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGAAGTGGCCACGTACA
Hairpin Sequence:
5'-CCGG-CCTTGGAAGTGGCCACGTACA-CTCGAG-TGTACGTGGCCACTTCCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGAAGTGGCCACGTACACTCGAGTGTACGTGGCCACTTCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGAAGTGGCCACGTACACTCGAGTGTACGTGGCCACTTCCAAGG-3'

Other clones with same target sequence:

(none)