Construct: shRNA TRCN0000199320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002513.2-661s1c1
DNA Barcode:
CGCTGGGCACTGGCTGTATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME3 (4832)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 3
Transcript:
RefSeq NM_002513.2 (CURRENT)
Match location:
Position 661 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGGGCACTGGCTGTATGA
Hairpin Sequence:
5'-CCGG-CGCTGGGCACTGGCTGTATGA-CTCGAG-TCATACAGCCAGTGCCCAGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGGGCACTGGCTGTATGACTCGAGTCATACAGCCAGTGCCCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGGGCACTGGCTGTATGACTCGAGTCATACAGCCAGTGCCCAGCG-3'

Other clones with same target sequence:

(none)