Construct: shRNA TRCN0000199383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002953.3-2510s1c1
DNA Barcode:
CTAATGAGGGTGTGAGAAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KA1 (6195)
Gene Description:
ribosomal protein S6 kinase A1
Transcript:
RefSeq NM_002953.3 (NON-CURRENT)
Match location:
Position 2510 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAATGAGGGTGTGAGAAGTG
Hairpin Sequence:
5'-CCGG-CTAATGAGGGTGTGAGAAGTG-CTCGAG-CACTTCTCACACCCTCATTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAATGAGGGTGTGAGAAGTGCTCGAGCACTTCTCACACCCTCATTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAATGAGGGTGTGAGAAGTGCTCGAGCACTTCTCACACCCTCATTAG-3'

Other clones with same target sequence:

(none)