Construct: shRNA TRCN0000199391

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001129206.1-369s1c1
DNA Barcode:
CCTGGACATTTCCGAGGTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA1639 (57729)
Gene Description:
KIAA1639 protein
Transcript:
RefSeq XM_001129206.1 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199391)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGACATTTCCGAGGTGGA
Hairpin Sequence:
5'-CCGG-CCTGGACATTTCCGAGGTGGA-CTCGAG-TCCACCTCGGAAATGTCCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGACATTTCCGAGGTGGACTCGAGTCCACCTCGGAAATGTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGACATTTCCGAGGTGGACTCGAGTCCACCTCGGAAATGTCCAGG-3'

Other clones with same target sequence:

(none)