Construct: shRNA TRCN0000199483

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002734.3-1135s1c1
DNA Barcode:
GCACTACTGATGAATCGTCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR1A (5573)
Gene Description:
protein kinase cAMP-dependent type I regulatory subunit alpha
Transcript:
RefSeq NM_002734.3 (NON-CURRENT)
Match location:
Position 1135 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199483)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTACTGATGAATCGTCCT
Hairpin Sequence:
5'-CCGG-GCACTACTGATGAATCGTCCT-CTCGAG-AGGACGATTCATCAGTAGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTACTGATGAATCGTCCTCTCGAGAGGACGATTCATCAGTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTACTGATGAATCGTCCTCTCGAGAGGACGATTCATCAGTAGTGC-3'

Other clones with same target sequence:

(none)