Construct: shRNA TRCN0000199486

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004606.2-5776s1c1
DNA Barcode:
GCCTAGGTGGTTCACCTTTCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAF1 (6872)
Gene Description:
TATA-box binding protein associated factor 1
Transcript:
RefSeq NM_004606.2 (NON-CURRENT)
Match location:
Position 5776 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199486)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGGTGGTTCACCTTTCC
Hairpin Sequence:
5'-CCGG-GCCTAGGTGGTTCACCTTTCC-CTCGAG-GGAAAGGTGAACCACCTAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGGTGGTTCACCTTTCCCTCGAGGGAAAGGTGAACCACCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGGTGGTTCACCTTTCCCTCGAGGGAAAGGTGAACCACCTAGGC-3'

Other clones with same target sequence:

  1. TRCN0000280259