Construct: shRNA TRCN0000199565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002821.3-3740s1c1
DNA Barcode:
GCCACTCATCTGCCAACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK7 (5754)
Gene Description:
protein tyrosine kinase 7 (inactive)
Transcript:
RefSeq NM_002821.3 (NON-CURRENT)
Match location:
Position 3740 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACTCATCTGCCAACTTTG
Hairpin Sequence:
5'-CCGG-GCCACTCATCTGCCAACTTTG-CTCGAG-CAAAGTTGGCAGATGAGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACTCATCTGCCAACTTTGCTCGAGCAAAGTTGGCAGATGAGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACTCATCTGCCAACTTTGCTCGAGCAAAGTTGGCAGATGAGTGGC-3'

Other clones with same target sequence:

(none)