Construct: shRNA TRCN0000199595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003681.2-1087s1c1
DNA Barcode:
GACCCAGAGATCGTCGTCCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDXK (8566)
Gene Description:
pyridoxal kinase
Transcript:
RefSeq NM_003681.2 (NON-CURRENT)
Match location:
Position 1087 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCAGAGATCGTCGTCCAG
Hairpin Sequence:
5'-CCGG-GACCCAGAGATCGTCGTCCAG-CTCGAG-CTGGACGACGATCTCTGGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCAGAGATCGTCGTCCAGCTCGAGCTGGACGACGATCTCTGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCAGAGATCGTCGTCCAGCTCGAGCTGGACGACGATCTCTGGGTC-3'

Other clones with same target sequence:

(none)