Construct: shRNA TRCN0000199625

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002019.2-5483s1c1
DNA Barcode:
GAGCATTAGCTGGCGCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT1 (2321)
Gene Description:
fms related tyrosine kinase 1
Transcript:
RefSeq NM_002019.2 (NON-CURRENT)
Match location:
Position 5483 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199625)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCATTAGCTGGCGCATATT
Hairpin Sequence:
5'-CCGG-GAGCATTAGCTGGCGCATATT-CTCGAG-AATATGCGCCAGCTAATGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCATTAGCTGGCGCATATTCTCGAGAATATGCGCCAGCTAATGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCATTAGCTGGCGCATATTCTCGAGAATATGCGCCAGCTAATGCTC-3'

Other clones with same target sequence:

(none)