Construct: shRNA TRCN0000199638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001823.3-1250s1c1
DNA Barcode:
GCCCTGCTGCTTCCTAACTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKB (1152)
Gene Description:
creatine kinase B
Transcript:
RefSeq NM_001823.3 (NON-CURRENT)
Match location:
Position 1250 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGCTGCTTCCTAACTTA
Hairpin Sequence:
5'-CCGG-GCCCTGCTGCTTCCTAACTTA-CTCGAG-TAAGTTAGGAAGCAGCAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGCTGCTTCCTAACTTACTCGAGTAAGTTAGGAAGCAGCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGCTGCTTCCTAACTTACTCGAGTAAGTTAGGAAGCAGCAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000350437