Construct: shRNA TRCN0000199648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000269.2-500s1c1
DNA Barcode:
GCTTGTGGTTTCACCCTGAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NME1 (4830)
Gene Description:
NME/NM23 nucleoside diphosphate kinase 1
Transcript:
RefSeq NM_000269.2 (CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTGGTTTCACCCTGAGG
Hairpin Sequence:
5'-CCGG-GCTTGTGGTTTCACCCTGAGG-CTCGAG-CCTCAGGGTGAAACCACAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTGGTTTCACCCTGAGGCTCGAGCCTCAGGGTGAAACCACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTGGTTTCACCCTGAGGCTCGAGCCTCAGGGTGAAACCACAAGC-3'

Other clones with same target sequence:

(none)