Construct: shRNA TRCN0000199711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002596.2-1351s1c1
DNA Barcode:
GCACCTCATCTTTCGCCTCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDK18 (5129)
Gene Description:
cyclin dependent kinase 18
Transcript:
RefSeq NM_002596.2 (NON-CURRENT)
Match location:
Position 1351 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCTCATCTTTCGCCTCCT
Hairpin Sequence:
5'-CCGG-GCACCTCATCTTTCGCCTCCT-CTCGAG-AGGAGGCGAAAGATGAGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCTCATCTTTCGCCTCCTCTCGAGAGGAGGCGAAAGATGAGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCTCATCTTTCGCCTCCTCTCGAGAGGAGGCGAAAGATGAGGTGC-3'

Other clones with same target sequence:

(none)