Construct: shRNA TRCN0000199718

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024619.2-1174s1c1
DNA Barcode:
GTATGAGCAGAGGGATGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FN3KRP (79672)
Gene Description:
fructosamine 3 kinase related protein
Transcript:
RefSeq NM_024619.2 (NON-CURRENT)
Match location:
Position 1174 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000199718)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTATGAGCAGAGGGATGTATG
Hairpin Sequence:
5'-CCGG-GTATGAGCAGAGGGATGTATG-CTCGAG-CATACATCCCTCTGCTCATAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTATGAGCAGAGGGATGTATGCTCGAGCATACATCCCTCTGCTCATACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTATGAGCAGAGGGATGTATGCTCGAGCATACATCCCTCTGCTCATAC-3'

Other clones with same target sequence:

(none)